This Project was dedicated to the development of a new volumetric disinfection technology based on a specific micro aerosol generated from neutral EAS. This was the first time when EAS was utilized as the micro aerosol disseminated within contained environment for it’s thoroughly disinfection. Therefore, a toxicity of micro aerosol generated from neutral Electrochemically Activated Solutions (MAEAS) was studied with animals positioned in the environment filled with MAEAS.